The invention relates to the field of electric digital data processing, and in particular relates to a generating method and system of a three-dimensional simulation training case of a power grid substation. The generating method comprises the following steps of: generating the training case including a initial substation state and an operation step list; updating the initial substation state of the training case according to a received initial user substation state; generating a three-dimensional simulation scene, receiving a plurality of user operation steps in sequence, and updating the operation step list of the training case according to the received user operation steps; and storing the updated training case into the three-dimensional simulation training system. By utilizing the generating method of the three-dimensional simulation training case of the power grid substation, according to the requirements of the training case, a training case is established, case data of a teaching plan are automatically generated through state setting and demonstration operation in a three-dimensional simulation system of the substation, procedures of three-dimensional animation production, manual file edition and configuration or training system modification are not required, and the working amount during the generation of the cases is effectively reduced.