The invention discloses a rotational mating breeding method for pinctada fucata groups. The method comprises the following steps of: collecting at least three pinctada fucata groups from different sources, and constructing an F1 generation diallel crossing group; before a breeding season, randomly sampling over 1,000 individuals from every each group for performing character measurement, computing a breeding character average value or a multi-character comprehensive selection index, sequencing according to sizes, and at least selecting five groups serving as cone breeding groups by adopting a last-one-out system; selecting largest female and male individuals from every each selected group, mating male individuals of a No.1 group with female individuals of a No.2 group, mating male individuals of a No.2 group with female individuals of a No.3 group, and so on, and rotationally mating, wherein the ratio of female individuals to male individuals is 2:1, and at least four male individuals are selected; and breeding generation after generation to over five generations. Due to the adoption of the method, high selection strength is realized, genetic gain is increased, and the genetic improvement of every each generation of the bred pinctada fucata can be over 15 percent.