The invention discloses a traditional Chinese medicine multicomponent micro-emulsion composition having a function of resisting lung cancer, a preparation method and application thereof in preparation of an orally-taking lung cancer resistant medicine. The application comprises the following steps of: adding ganoderma lucidum total triterpenes, coixenolide, ganoderan and semen coicis polysaccharide used as raw material medicines into a nano-medicine composition which consists of surfactants and cosurfactant, wherein the traditional Chinese medicine multicomponent micro-emulsion composition comprises the following components in parts by weight: 5-30 parts of ganoderma lucidum total triterpenes, 45-150 parts of coixenolide, 5-50 parts of ganoderan and 10-90 parts of semen coicis polysaccharide, 45-200 parts of surfactants, 20-100 parts of cosurfactant and 300-900 parts of distilled water. The invention also discloses a preparation method of the composition. The ganoderma lucidum total triterpenes and the coixenolide are carried into a non-water phase, and the lucid ganoderma and the semen coicis polysaccharide are carried into a water phase, so that the coixendide can be used as medicines and also can be used as an auxiliary; the polysaccharide can enhance the immunization and the stability of a micro-emulsion system; and by the micro-emulsion, the absorption of the ganoderma lucidum total triterpenes and coixenolide used as the lipid solubility components can be increased, and the matching of four components have a synergy anti-tumor effect so as to increase the anti-tumor effect.