The invention relates to the field of a Chinese traditional medicine extraction, and particularly relates to an extraction process of radix puerariae and a measuring method of puerarin and total puerariae flavones. The extraction process sequentially comprises the steps of two-time alcohol extraction, resin absorption, alcohol concentration and recovery, normal butyl alcohol extraction, concentration drying, and the like. According to the invention, through comparison among different extraction methods, a more appropriate extraction method, namely alcohol extraction is screened out, then the various influence factors of in alcohol extraction of radix puerariae are tested by adopting an orthogonal experimental method, data analysis is carried out by using a multi-index comprehensive scoring method to determine the optimal condition of radix puerariae extraction, which is shown in the specification of the inveniton, thereby providing the basis for developing and utilizing radix puerariae resources.