The invention discloses an enzyme-assistant extraction technology of hyperin in leaves of Acanthopanax senticosus Harms. The technology comprises the following steps: taking crude powder of leaves of Acanthopanax senticosus Harms, adding water having a mass 1-2 times higher than the crude powder, adding a mixture of cellulase and pectase until the concentration of the mixture reaches 0.2-0.3mg/ml, adjusting the pH of the obtained solution to 4.0-5.0, heating to 40-60DEG C, carrying out enzymatic hydrolysis for 1.5-2h, heating to 70-80DEG C, and inactivating, wherein the mass ratio of the cellulase to the pectase of the mixture is (2-3):1; adding 90-95% ethanol, immersing, carrying out percolating extraction, collecting the obtained percolating liquid, and recovering ethanol to obtain a concentrate; diluting the concentrate by adding water having a mass 1-2 times higher than the concentrate, and filtering to obtain a supernatant; adding the supernatant into a macro-porous adsorption resin, eluting by water, eluting by 70-75% ethanol, collecting the obtained eluate, recovering ethanol, and carrying out reduced pressure drying to obtain concentrate powder; and carrying out reversed-phase resin column chromatography of the concentrate powder, eluting by 20-30% ethanol, collecting the obtained eluate, recovering ethanol to obtain crude crystals, and re-crystallizing methanol to obtain the hyperin. The technology, which adopts leaves of Acanthopanax senticosus Harms as a raw material, adopts the enzyme-assistant extraction method and adopts ethanol as a solvent to elute, has the advantages of environmental protection, no pollution, convenient operation and production cost reduction.