The invention provides a preparation method for total lignans in hawthorn seeds. The method comprise the steps of smashing a raw material of hawthorn seeds; extracting by using ethanol-water with a certain concentration; concentrating under reduced pressure to obtain a crude material; suspending with water; loading a water phase on a macroporous resin directly; and eluting by an ethanol-water system to obtain an extract of the total lignans. The extract is the effective portion having pharmacological activity in the hawthorn seeds. The total lignans in the hawthorn seeds provided by the invention has obvious antineoplastic activity; and the hawthorn is a plant having homology of medicine and food, without toxic and side effect, so that the total lignans can be developed into a novel antineoplastic drug. The total lignans in the hawthorn seed can be made into various oral preparations. The method is reasonable in design, and has the advantages of simple process, low cost, low energy consumption and low pollution in a production process.