A flotation separation method of antimony sulfide gold minerals comprises the steps of performing bulk flotation to obtain antimony-gold mixed concentrate firstly and then separating the antimony-gold mixed concentrate to obtain antimony concentrate and gold concentrate, wherein in the bulk flotation, lead nitrate is taken as an activator for the antimony gold minerals, while sodium n-butylxanthate is used as a collecting agent and 2# oil is taken as a foaming agent; and in the flotation separation process, active carbon is used for reagent removal, while sodium hydroxide is used for adjusting the pH value to be 8.5-9.5, sodium humate and starch are used as a gold-containing mineral inhibitor and N, N-sodium diethyldithiocarbamate is used as the antimony mineral collecting agent. According to the flotation separation method, the antimony sulfide minerals can be effectively separated from the gold-carrying minerals through flotation, so that the antimony concentrate having the antimony grade of 33.82%-37.28% and the recovery rate of 87.21%-91.62% is obtained, and simultaneously, the gold concentrate having the grade of 40.27-45.81g/ton and the recovery rate of 85.64%-92.16% is obtained, through bulk flotation and further separation under the conditions that the feeding antimony grade is 0.37%-0.87% and the gold grade is 1.86-3.26 g/ton.