The present invention relates to microvesicles derived from a protoplast which is a bacterial, archaea, fungal or plant cell or the like from which a cell wall is removed. The microvesicles derived from a protoplast of the present invention enables free loading of a material necessary for diagnosis, treatment, vaccine, target induction, cell membrane fusion with a target cell, reduction of in vivo and in vitro side effects, stability improvement, and the like, and allows the therapeutic material, the diagnostic material and/or the vaccine material to be delivered specifically to a specific tissue or cell.