The invention relates to a regeneration method for a rosa wichuriana plant taking leaves as explant, which comprises the following steps: cultivating an aseptic seedling in a subculture medium in advance, cultivating a callus in an induction medium, cultivating and differentiating adventitious buds in a proliferation and differential culture medium, cultivating and regenerating a plant in a seedling culture medium, and finally obtaining a complete plant in a rootage culture medium. The method has the advantages of wide explant sources and convenience in material fetching. The obtained embryogenic callus can be a favorable acceptor for agrobacterium mediated genetic transformation, and can also be used for the research on genetic transformation of rosa wichuriana. The invention further relates to the culture medium composition for the method.