The invention discloses a graphene glue film, a preparation method of the graphene glue film and a transfer method of graphene. According to the preparation method, polyvinyl formal adhesive is coated on a graphene film which grows well on a substrate in a spin-on way, thus a large-scale, less-defect and self-supported transparent graphene glue film can be prepared; the graphene glue film can be widely transferred in a fixed direction; the operation is simple; few requirements are brought to the condition; and as shown in the Raman spectrum, electrical measurement and magnetic measurement and methods the like, the method disclosed by the invention can be carried out to prepare high-quality graphene, and large-scale and less-defect graphene film can be transferred, and as a result, a standard graphene characteristic curve can be obtained. The graphene glue film prepared by the method disclosed by the invention is convenient and quick to store and transfer, has the capacity of quickly measuring the performance of the graphene, is simple and quick in process, low in transfer cost, and suitable for manual shearing and automatic shearing under large-scale machines, can provide guarantee to the high-quality of the graphene film, and can greatly promote the application and popularization of the graphene materials.