The invention relates to a preparation method of anti-wrinkle real silk fabrics in a hollow structure. According to the preparation method, centre yarns and cocoon silks in a moist state in a reeling silk bath are coated together, water soluble fibers of the centre yarns in core covering silks are dissolved through refining, and the manufacture of hollow real silk is completed at the reeling silk stage. The preparation method has the advantages that the production cost is low, the process is simple, the hollow real silk has the elasticity, the anti-wrinkle recovery performance of the real silk is enhanced, the anti-wrinkle performance of finished products is high, the wrinkling cannot be generated after washing, and the wide application can be realized.