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发明授权
CN103060447B 四种菌的三重实时荧光PCR检测引物、探针、检测试剂盒和检测方法 有权

四种菌的三重实时荧光PCR检测引物、探针、检测试剂盒和检测方法
摘要：
本发明公开了一种猪霍乱沙门氏菌、丙型副伤寒沙门氏菌、伤寒沙门氏菌及鸡伤寒沙门氏菌三重实时荧光PCR检测引物、探针、检测试剂盒和检测方法。本发明利用三重实时荧光PCR方法，将猪霍乱沙门氏菌、丙型副伤寒沙门氏菌共有序列用于检测猪霍乱沙门氏菌、丙型副伤寒沙门氏菌，伤寒沙门氏菌的特异序列用于检测伤寒沙门氏菌，鸡伤寒沙门氏菌的特异序列用于检测鸡伤寒沙门氏菌，通过一次实时荧光PCR扩增来判断样品中是否受到猪霍乱沙门氏菌、丙型副伤寒沙门氏菌、伤寒沙门氏菌及鸡伤寒沙门氏菌的污染，再通过单一荧光PCR扩增区分猪霍乱沙门氏菌和丙型副伤寒沙门氏菌，检测快速，可在31h完成从制备样品到出具检测结果的过程，结果可靠，且灵敏度高、特异性强。
摘要（英）：

The invention discloses primers, probes, a test kit and a test method for triple real-time fluorescence PCR detection of Salmonella choleraesuis, Salmonella paratyphi, Salmonella typhi, and Salmonella gallinarum. According to the present invention, a triple real-time PCR method is used, wherein a consensus sequence of Salmonella choleraesuis and Salmonella paratyphi is used for the detection of Salmonella choleraesuis and Salmonella paratyphi, a specific sequence of Salmonella typhi is used for the detection of Salmonella typhi, and a specific sequence of Salmonella gallinarum is used for the detection of Salmonella gallinarum. Whether a sample is contaminated by Salmonella choleraesuis, Salmonella paratyphi, Salmonella typhi, and Salmonella gallinarum is determined via a real-time fluorescence PCR amplification, and then Salmonella choleraesuis and Salmonella paratyphi are distinguished via a single fluorescent PCR amplification; the detection is rapid, the process of preparing sample and issuing test results can be finished in 31hours, the results are reliable, and sensitivity and specificity are high.

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