The invention provides a video quality acquisition method. The video quality acquisition method comprises the steps of receiving videos to be processed, acquiring significance detecting results of video frame layers, acquiring significance detecting results of video sequence layers, and acquiring video quality information according to the significance detecting results of the video frame layers and the significance detecting results of the video sequence layers. According to the video quality acquisition method, due to the fact that the videos to be processed are processed, the significance detecting results of the video frame layers and the significance detecting results of the video sequence layers can be obtained, the video quality information can be obtained according to the significance detecting results of the video frame layers and the significance detecting results of the video sequence layers, and therefore video quality results are integrated with the significance of the video frame layers and the video sequence layers, and the obtained video quality information is more suitable for human eye sensing results and more accurate.