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发明公开
CN103074333A 与小麦抗条锈病基因相连锁的分子标记、其获得方法及应用 有权

与小麦抗条锈病基因相连锁的分子标记、其获得方法及应用
摘要：
本发明涉及农业生物技术工程和小麦抗病遗传育种领域，具体地涉及与小麦抗条锈病基因相连锁的分子标记、其获得方法及应用。所述与小麦抗条锈病基因相连锁的分子标记是通过使用简并引物：上游引物：5’-AAGTGGAACAAGGTTACG-3’；下游引物：5’-GGIGGIGTIGGIAAIACIAC-3’扩增而得。该标记可用于小麦抗条锈病的辅助选择育种，可克服常规育种中小麦条锈病抗性鉴定易受环境影响、稳定性和重复性较差、工作量大、费时费力、育种周期长等缺点，进而加快小麦抗条锈病品种的育种进程。
摘要（英）：

The invention relates to the fields of agricultural biotechnical engineering and wheat disease resistance genetic breeding, and in particular relates to a molecular marker interlocking with a wheat stripe rust resistant gene, an acquisition method and application thereof. The marker interlocking with the wheat stripe rust resistant gene is obtained through amplification by degenerate primers: an upstream primer 5-'AAGTGGAACAAGGTTACG-3', and a downstream primer 5'-GGIGGIGTIGGIAAIACIAC-3'. The marker can be used to assist selective breeding of wheat stripe rust resistance, and overcome the shortcomings in wheat stripe rust resistance identification in conventional breeding, such as vulnerability to environment impact, poor stability and repeatability, heavy workload, time and labor consumption and long breeding cycle, so as to speed up the breeding process of a wheat stripe rust resistance variety.

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