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CN103119053A 用于生产能够转化阿拉伯糖的细胞的方法 无效 - 撤回

用于生产能够转化阿拉伯糖的细胞的方法
摘要：
本发明涉及用于生产能够转化阿拉伯糖的细胞的方法，其包含下述步骤：a)将基因AraA、araB和araD引入不能够转化阿拉伯糖的宿主菌株，该细胞被命名为构建细胞；b)使构建细胞进行适应性进化直到获得转化阿拉伯糖的细胞；c)任选地，使第一阿拉伯糖转化细胞进行适应性进化，以改进阿拉伯糖转化；在步骤b)或c)中生产的细胞被命名为第一阿拉伯糖转化细胞；d)分析第一阿拉伯糖转化细胞和构建细胞的全基因组或部分基因组；e)鉴定第一阿拉伯糖转化细胞中的单核苷酸多态性(SNP)；和f)在能够转化阿拉伯糖的细胞的合理设计中使用SNP的信息；g)构建在步骤f)中设计的能够转化阿拉伯糖的细胞。
摘要（英）：

The invention relates to a process for the production of cells which are capable of converting arabinose, comprising the following steps: a)Introducing into a host strain that cannot convert arabinose, the genes AraA, araB and araD, this cell is designated as constructed cell; b)Subjecting the constructed cell to adaptive evolution until a cell that converts arabinose is obtained, c)Optionally, subjecting the first arabinose converting cell to adaptive evolution to improve the arabinose conversion; the cell produced in step b) or c) is designated as first arabinose converting cell; d)Analysing the full genome or part of the genome of the first arabinose converting cell and that of the constructed cell; e)Identifying single nucleotide polymorphisms (SNP's) in the first arabinose converting cell; and f)Using the information of the SNP's in rational design of a cell capable of converting arabinose; g)Construction of the cell capable of converting arabinose designed in step f).

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