The invention discloses a seed introduction method of submerged plant vallisneria, which comprises the steps of collecting ripening fruits of the vallisneria, removing epicarp, obtaining seeds, washing the seeds with clean water within 0-24h to keep the seeds moist, transporting the collected seeds to a destination by a water-culture method, mixing the seeds transported to the destination with moist river sand, refrigerating the seeds at 0-10 DEG C, preserving the seeds by shading above 95% till complete darkness, ventilating and replenishing water frequently, maintaining a moisture content of each seed to be 35-50%, sowing the preserved seeds in a mixed matrix, a soil matrix or a yellow clay matrix, controlling a temperature to be 10-30 DEG C, the illumination intensity to be 800-3500LUX, and the everyday illumination time to be 10-12h, allowing the seeds to germinate and grow, obtaining germinated seedlings, planting the germinated seedlings in the mixed matrix with a 10-15cm water level for culturing, managing other conditions according to a convention, planting the vallisneria, and allowing the vallisneria to grow. The mixed matrix comprises the following components by total mass fraction: 20-40% of sand and 60-80% of soil. The seed introduction method is long in seed preservation time, high in germination rate, and high in emergence rate and planting survival rate.