The invention discloses a cycloartane type triterpene compound with a novel structure and an application thereof in preparing an anti-tumor medicine. The result of targeted screening of tumor specific mutant gene products indicates that the cycloartane type triterpene compound has anti-tumor activity against the tumor cells carrying tumor specific mutant genes p53 and Ras and does not have or only has low cytotoxic activity against normal cells, wherein KY41 can activate the cancer suppressor gene p16; and the characteristic indicates that the cycloartane type triterpene compound and a medicinal composition taking the same as an effective treatment dose have application potential in preparing individual-based treatment medicines for the tumor specific mutant genes p53 and Ras and the cancer suppressor gene p16.