The invention discloses a polypeptide separated from a centipede zymolyte and measures the anti-thrombus property thereof. A polypeptide consisting of seven amino acids is separated and purified from a centipede zymolyte by the separation methods such as gel chromatography, reversed-phase high-performance liquid chromatography and the like; the sequence of the polypeptide is Asp-Leu-Asp-His-Tyr-Ser-Phe according to measurement of an Edman degradation method; and the molecular weight of the polypeptide is 895.4Da according to measurement of mass spectrography. The anti-thrombus activity of the polypeptide is measured by measuring APTT and PT as well as the anti-platelet aggregation activity. The polypeptide disclosed by the invention can be used for delaying the APTT time and realizing an effect of inhibiting platelet aggregation, has anti-thrombus activity and can be used for preparing an anti-thrombus medicine.