The invention discloses a tobacco flavor with sweetening function and an application of the tobacco flavor. The tobacco flavor is prepared by using ocimum basilicum L. as raw material and extracting by using combination of supercritical CO2 (carbon dioxide) extraction technology and molecular distillation technology. The tobacco flavor comprises the following main components: linalool, farnesene, linalool, camphor, pinene, camphene, ocimene, terpineol, citronella, eugenol and the like. The tobacco flavor after being added into tobacco has the functions in enriching tobacco fragrance, increasing sweet and exquisite affects, enhancing fragrance quality, reducing simulation, enabling the tobacco fragrance to be more permeable and enhancing comfort.