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CN103146642A 一种定向诱导间充质干细胞分化的方法 无效 - 驳回

一种定向诱导间充质干细胞分化的方法
摘要：
本发明公开了一种定向诱导间充质干细胞分化的方法。本发明提供的定向诱导间充质干细胞分化的方法，包括如下步骤：将离体的诱导细胞接种并吸附于聚碳酸酯膜的下表面，将离体的间充质干细胞接种至所述聚碳酸酯膜的上表面，然后将所述诱导细胞和所述间充质干细胞共培养，促使所述间充质干细胞分化为目标细胞；所述间充质干细胞和所述诱导细胞均不能通过所述聚碳酸酯膜的孔径。UCMSCs向表皮细胞的分化在未来能够为临床皮肤再生的研究的进展起到有益作用。本发明为增加MSCs多向分化的诱导方法开拓思路，为进一步采用UCMSCs为种子细胞构建组织工程皮肤提供依据。
摘要（英）：

The invention discloses a method for the directional induction of the differentiation of MSCs. The method for the directional induction of the differentiation of MSCs comprises the following steps: inoculating and adsorbing in-vitro induction cells to the lower surface of a polycarbonate film, inoculating in-vitro MSCs to the upper surface of the polycarbonate film, and co-culturing the induction cells and the MSCs to promote the differentiation of the MSCs to target cells, wherein each of the MSCs and the induction cells cannot pass through the holes of the polycarbonate film. The differentiation of UCMSCs to cuticle cells has a beneficial effect on the clinic skin regeneration research progress in the future. The method develops ideas for the increase of the induction method for the multi-directional differentiation of the MSCs, and provides bases for the construction of tissue engineering skins through adopting the UCMSCs as seed cells.

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