The invention discloses a manufacturing method of an oscilloscope tube capable of displaying motion trails of electrons. The manufacturing method comprises the following technical steps of (1), display of an electric signal: emitting electrons through an electronic emitting system, focusing the electrons by an electrostatic focusing system, and displaying a focused electron beam on a fluorescent screen, wherein an electronic gun is at an electrostatic focusing and electrostatic deflecting mode; and (2), display of the motion trails of the electrons: firstly, vacuumizing the oscilloscope tube to be at a high vacuum state and decomposing and activating a cathode at the high vacuum state; secondly, after finishing the operations of the step, filling inert gas of 1*10<-3>-10Pa into the oscilloscope tube; and finally, filling different inert gases according to different colors. The manufacturing method of the oscilloscope tube capable of displaying the motion trails of the electrons, disclosed by the invention, has the advantages that when a signal is inputted into the oscilloscope tube, the motion trail of the electronic beam in vacuum is observed on one aspect, the waveform of the electric signal is synchronously displayed on the fluorescent screen of the oscilloscope tube on the other aspect; and the manufactured oscilloscope tube can be applied to teaching demonstration of junior high schools, senior high schools and electron junior colleges, and is used for demonstrating a deflection situation generated by the electronic beam under the action of an electric field force or a magnetic field force in an electric field or a magnetic field.