The invention discloses novel lanostane triterpenoids with two novel structures and an application of the novel lanostane triterpenoids in preparation of antitumor activity pharmaceuticals. A targeted screening result aiming at tumor specific mutant gene products shows that the two novel lanostane triterpenoids with similar structures have cytotoxic activity on tumor cells with tumor specific mutant genes p53 and Ras, and do not have or only have low cytotoxic activity on normal cells, the characteristic shows that the novel lanostane triterpenoids and therapeutically effective amounts of pharmaceutical compositions thereof have application potential on preparation of individual-based treatment pharmaceuticals for tumor specific mutant genes p53 and Ras.