The invention provides a microalgae grease extraction method which comprises the following steps: regulating the pH value of a microalgae solution, heating, pressurizing, and then returning to the normal pressure state; and adding biological enzymes, and mixing, wherein the biological enzymes are added in two steps, non-proteinase is firstly added, and proteinase is secondly added. According to the method provided by the invention, when the biological enzymes are used for treating wet algae, the proteinase is added after the non-proteinase acts for some time, so that the non-proteinase can not be decomposed or destroyed by the proteinase, thereby greatly increasing the wall-breaking efficiency. Compared with the method of simultaneously adding the proteinase and the non-proteinase, the wall-breaking efficiency is obviously increased, thereby obviously increasing the grease extraction rate and yield.