The invention discloses a prescription composition and a preparation method of gefitinib liposome, and discloses the influences of preparation total ethanol content on liposome encapsulation efficiency, particle size distribution, stability and anti-tumor effect. With the preparation method provided by the invention, gefitinib liposome with an encapsulation efficiency higher than 90% can be prepared; when ethanol content in a final preparation is controlled at a value no higher than 10%, liposome relevant parameters are almost not changed. Therefore, a reference is provided for ethanol content control in gefitinib liposome preparation and industrial production.