The invention relates to a pseudoginsenoside RT6 and an extraction method thereof, and applications of pseudoginsenoside RT6 drug, belonging to new compounds and applications of new compound drugs. The extraction method comprises the following steps of: by taking panax quinquefolium stem leaves as raw materials, performing alcohol extraction, and positive and reverse phase silica gel column chromatography, and recrystallizing to obtain a new compound, wherein the chemical name of the new compound is (20S, 24R)-6-O-beta-D-glucopyranose-dammar-3-keto-20,24-epoxy-6alpha,12beta,25-triol. The pseudoginsenoside RT6 has wide drug applications in preparing anti-tumor drugs.