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发明公开
CN103196844A 液体植酸酶稳定性的测定方法 无效 - 驳回

液体植酸酶稳定性的测定方法
摘要：
本发明涉及一种测定液体植酸酶稳定性能的方法，该方法包括：1)将液体植酸酶在6℃～60℃的温度震荡处理10分钟～120小时；2)在步骤1)的处理过程中选取至少一个时间点测定该液体植酸酶震荡前后的吸光度和酶活；3)根据步骤2)中的吸光度的变化评价澄清度的稳定性，以及根据步骤2)中酶活的变化评价酶活的稳定性。该方法提供一种可以有效地定量比较各种液体植酸酶的稳定性能优劣的方法，特别是提供一种通过比较震荡前后液体植酸酶的吸光度和酶活活性的变化来判断不同液体植酸酶稳定性的优劣的方法。
摘要（英）：

The invention relates to a method for determining liquid phytase stability. The method comprises the steps that: (1) liquid phytase is subjected to an oscillation treatment for 10min-120h under a temperature of 6-60 DEG C; (2) during the treatment process in the step (1), at least one time point is selected for determining light absorbance and enzyme activities of the liquid phytase before and after oscillation; (3) clarity stability is evaluated according to the change in light absorbance obtained in the step (2); and (3) enzyme activity stability is evaluated according to the change in enzyme activities obtained in the step (2). According to the invention, a method for effectively and quantitatively comparing stabilities of various liquid phytase is provided. Specially, a method for determining stabilities of various liquid phytase through comparing the changes in light absorbance and enzyme activities of different liquid phytase before and after oscillation is provided by the invention.

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