The invention discloses a medicinal composition with a dysentery stopping effect and a preparation method thereof, and belongs to the technical field of livestock medicaments. The medicinal composition is prepared from mahonia, majoram, Chinese pulsatilla root, hawthorn, terminalia fruit, ginger, pomegranate husk charcoal and the like serving as raw medicaments, wherein the raw medicaments are treated by water extraction, 60 percent ethanol reflux and extraction and direct crushing respectively according to different properties of the medicaments and prepared into powder or granules. The medicinal composition has the effects of clearing away heat, cooling blood, detoxifying and stopping dysentery, and is mainly used for treating the dysentery of livestock infection.