The invention discloses a preparation method of a pistacia weinmannifolia spice for cigarettes, which comprises the following steps of: carrying out extraction on pistacia weinmannifolia leaves, branches or barks which are dried and crushed to 5-20 meshes by using an accelerated solvent extraction method so as to obtain a pistacia weinmannifolia leaf crude; putting the pistacia weinmannifolia leaf crude into a molecular distillation device under the condition of a high vacuum degree to carry out distillation; collecting a heavy-component separation product; and dissolving the heavy-component separation product into ethanol and propylene glycol in a volume ratio of 70% so as to prepare the pistacia weinmannifolia spice for cigarettes. Through smoke panel test comparison, the pistacia weinmannifolia spice for cigarettes can significantly enhance the richness and exquisite feeling of smoke smell of cigarettes, and can mainly increase the faint scent, spicy flavor, wood flavor, fruity flavor and flower flavor of cigarettes; and the spice is downy in fragrance, blown and elegant in penetrative property, and has good compatibility with the smoke smell of cigarettes.