The invention relates to a comprehensive anti-misoperation system of an intelligent transformer station. The comprehensive anti-misoperation system of the intelligent transformer station comprises a computer five-prevention system, a temporary ground wire and a temporary ground wire pile and is characterized in that wireless code acquiring devices and wireless signal transmitting nodes are hung on the temporary ground wire and the temporary ground wire pile, wherein the wireless code acquiring devices are connected with the wireless signal transmitting nodes, the wireless signal transmitting nodes are connected with a ground wire managing device through a wireless network, and the ground wire managing device is in communicating connection with the computer five-prevention system. The comprehensive anti-misoperation system can effectively monitor states of the temporary ground wire and the temporary ground wire pile in real time and prevent misoperation.