The invention discloses a method for preparing tobacco spice by tobacco fragments and application of the spice. The method adopting the tobacco fragments as the materials comprises the following steps of: extracting by a polar solvent to prepare tobacco extract; mixing the tobacco extract with macroporous resin; carrying out static adsorption and separation treatment to obtain un-adsorbed hidden spice components; and eluting the macroporous resin by a strong polar solvent to obtain adsorbed spice enhancement components. According to sensory evaluation, the two components are shown to have the action of remarkably improving suction mouthfeel quality relative to the original tobacco extract. Within a range with adding amount of 0.01%-0.03%, the fragrance is remarkably lifted and improved without bitter and astringent harshness. According to the method for preparing tobacco spice by tobacco fragments, the tobacco fragments are used as the extracting materials, the tobacco leftovers are reasonably utilized, so that the tobacco spice is natural and safe, clear in mouthfeel, cost-saving and higher in application value.