The invention relates to novel catgut implantation at acupoint and a preparation method thereof, which belong to the technical field of textile engineering. The novel catgut implantation at acupoint is prepared by the following steps of: extracting natural macromolecular polysaccharide from Chinese herbal medicines rich in polysaccharide components, dissolving the macromolecular polysaccharide in a solvent to prepare a spinning solution, adding Western medicine components for spinning to obtain the catgut implantation with the diameter of 0.5mm-2mm and the length of 0.2cm-5cm, and sterilizing and packaging the catgut implantation. The prepared catgut implantation is easily degraded and absorbed in a human body and has high biocompatibility, the process is simple, the cost is low, and harmful substances are not generated.