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发明公开
CN103265665A 一种樟脑分子印迹聚合物及其整体柱和填充色谱柱的制备方法与应用 失效 - 权利终止

一种樟脑分子印迹聚合物及其整体柱和填充色谱柱的制备方法与应用
摘要：
本发明公开了一种樟脑分子印迹聚合物及其整体柱和填充色谱柱的制备方法与应用，制备方法是将模板分子樟脑溶解在致孔剂中，分别加入功能单体、交联剂、引发剂混合，在冰水浴中超声后通氮气，分别将该反应物注入不锈钢色谱柱和容器中，密封，放入50-60℃烘箱内反应12-24h，热引发得到分子印迹聚合物整体柱和聚合物。将该聚合物研磨、过筛后填入色谱柱中得到分子印迹聚合物填充色谱柱。将此填充色谱柱和整体柱接到液相色谱泵上清洗脱去模板分子，最后得到具有良好分离效果的色谱柱和整体柱。本方法分子识别性能好，本发明所提供的分子印迹聚合物作为一种液相色谱柱填料，可实现对精油中樟脑的分离、富集和纯化。
摘要（英）：

The present invention discloses a camphor molecular imprinted polymer, and a preparation method and application of a monolithic column and a packed column thereof. The preparation method includes dissolving a template molecule camphor in a pore-foaming agent, adding a functional monomer, a crosslinker and an initiator, mixing, treating by ultrasound in an ice-water bath, feeding nitrogen, injecting the reactant into a stainless steel chromatographic column and a container, sealing, placing in an oven of 50-60 DEG C to react for 12-24h, and initiating by heat to obtain a molecular imprinted polymer monolith column and the polymer. The polymer is ground, sieved and filled in a chromatographic column to get a molecular imprinted polymer packed column. The packed column and the monolithic column are connected with a liquid chromatography pump to remove the template molecules. Finally the chromatographic and the monolithic column with good separation effects are obtained. The method is good in molecular recognition. The molecular imprinted polymer provided by the invention as a liquid chromatographic column filler can realize separation, enrichment and purification for camphor in essential oil.

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