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发明公开
CN103266176A 动物源性食品中囊尾蚴PCR-DHPLC检测引物、试剂盒及检测方法 无效 - 驳回

动物源性食品中囊尾蚴PCR-DHPLC检测引物、试剂盒及检测方法
摘要：
本发明公开了一种囊尾蚴PCR-DHPLC检测引物、试剂盒及检测方法。首先针对囊尾蚴的基因序列，设计了检测用引物即SEQ ID NO.1～3，采用PCR-DHPLC法对动物源性食品中囊尾蚴的定性检测。本发明所述试剂盒包括浓度为5U/μL的Taq DNA聚合酶和PCR反应液、dNTP各2.5mM及SEQ ID NO.1～3各10μM。本发明所述方法可检至1个囊尾蚴样品的精度。并且检测耗时短，操作简捷，可以节省大量的人力物力，适合快速检测的要求。
摘要（英）：

The invention discloses cysticercus PCR-DHPLC (polymerase chain reaction-denaturing high performance liquid chromatography) detection primers, a kit and a detection method in animal-derived food. Primers SEQ ID NO.1-3 are designed according to the gene sequence of cysticercus, and a PCR-DHPLC method is utilized to carry out qualitative detection on the cysticercus in the animal-derived food. The kit comprises a 5U/mu L Taq DNA (deoxyribonucleic acid) polymerase, 2.5 mM of PCR reaction solution, 2.5 mM of dNTP (deoxyribonucleotide triphosphate), 10 mu M of SEQ ID NO.1, 10 mu M of SEQ ID NO.2 and 10 mu M of SEQ ID NO.3. The method disclosed by the invention can detect the cysticercus at the precision of 1 cysticercus sample. The invention has the advantages of short time consumption and simple operation, can save abundant human resources and material resources, and is suitable for the requirements of quick detection.

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