The invention discloses a bio-fuel burning machine which comprises a burner, an air duct and a main air chamber. The burner is communicated with one end of the air duct, the other end of the air duct is communicated with the main air chamber, and fire-resistant layers used for absorbing heat and heating are respectively arranged on the inner wall of the burner and/or the inner wall of the air duct. The bio-fuel burning machine has the advantages that the fire-resistant layers are respectively additionally arranged on the inner wall of the burner and/or the inner wall of the air duct and can effectively absorb and maintain the heat generated in a primary ignition procedure, so that the temperature required for burning bio-fuel in a follow-up procedure is guaranteed, and the bio-fuel can be ignited easily and can be burned sufficiently. The invention is applicable to burning machines.