The invention discloses a Chinese and western medicine compound preparation containing tilmicosin. The Chinese and western medicine compound preparation containing tilmicosin comprises the following components by mass percent: 2-30% of tilmicosin, 0.5-10% of taurine, 5-30% of milkvetch, 5-30% of isatis root, 5-30% of indigowoad leaf and the balance of officinal filling auxiliary material. The invention also discloses a preparation method of the Chinese and western medicine compound preparation containing tilmicosion. The Chinese and western medicine compound preparation containing tilmicosion has the advantages of stable effect, convenience in application and easiness in preparation when being used for treating respiratory diseases of beasts and birds.