The invention discloses a mycoplasma pneumonia detection kit. The mycoplasma pneumonia detection kit comprises outer primers F3 and B3 designed through loop-mediated isothermal amplification (LAMP) technology, inner primers FIP and BIP and a loop primer, wherein the sequences of the outer primers F3 and B3, the inner primers FIP and BIP and the loop primer are respectively shown as SEQ ID NO.1-SEQ ID NO.5. The primers adopted by the kit are good in specificity, high in accuracy and high in sensitivity; and the kit is simple to operate, can be used for fast detecting mycoplasma pneumoniae pneumonia, and provides guarantee for the clinical early-stage diagnosis and treatment.