The invention provides a device monitoring suspension of stimulated animal weightlessness tails in real time. The device comprises at least one box body unit, the box body unit comprises at least one tail suspending cage, a camera shooting device and a computer system, wherein the tail suspending cage is arranged in the box body unit and used for providing space for a suspension test on the animal tails; the camera shooting device is arranged above the box body unit and used for acquiring image information of the suspension test on the animal tails in the box body unit, a suspending device is arranged above each tail suspending cage and used for being connected with the animal tails, each tail suspending cage is provided with a falling-off detecting device which is used for detecting whether the animal tails fall off in the suspension test on the animal tails, and the falling-off detecting device gives out an alarming signal when the animal tails are detected to fall off; the computer system is used for receiving the image information transmitted by the camera shooting device and the alarming signal transmitted by the falling-off detecting device and transmitting the alarming signal to an appointed tester. The device can accurately acquire the state of autonomic action of the suspended animal tails and automatically give an alarm when the animal tails fall off.