A design method for a light source subsystem of a light environment simulation device comprises the following steps: 1, selecting light source arrangement way, and sequentially arranging a plurality of light source modules to form a round internal light source ring and a round external light source ring which are concentric, wherein optical axes of the light source modules on the internal light source ring deflect outwards in the radial direction of the internal light source ring, and each light source module comprises a light source and a hollow lamp shade in the shape of a round platform; 2, calculating the diameter of the external light source ring through the area of a simulation plane surface; and 3, acquiring the diameter of the internal light source ring, the optical axis deflection angle of the light source modules on the internal light source ring and the size of the lamp shade through the intensity of the illumination uniformity of the simulated light environment in the simulation plane surface area, the distance between the light environment simulation device and the simulation plane surface area, and the diameter of the external light source ring. Therefore, the method provided by the invention is lower in cost and higher in illumination uniformity.