The invention relates to the field of genetic transformation, and specifically relates to a method of establishing a regeneration system and a genetic transformation system of wild tomato solanum sities. The method comprises following steps: (1) solanum sitie aseptic seedlings are bred; (2) three different explants, namely the root, the stem, and leaves of solanum sities, are induced to produce callus tissue and multiple shoot points in a callus-inducing medium (CIM); (3) the induced multiple shoots are individually separated, then put in a aseptic seedling medium, and the multiple shoots are induced to generate roots and turn into seedlings in the aseptic seedling medium. Wild tomato solanum sities instead of modern technology clutivated tomatoes which have strong genotype specificity are chosen as the research objects, which benefit researches in tomato genetic engineering and the process in tomato molecular breeding. The built corresponding systems of solanum sities are suitable for the three explants, and the transformation medium and the culture conditions for the three explants are completely the same, therefore related research cost is largely reduced. More important, the method has advantages in that: the regeneration and transformation efficiencies are high, the system is stable and the testing time is short.