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发明公开
CN103320430A 用于p21靶基因相关miRNA的回收和鉴定的探针、试剂盒和方法 有权

用于p21靶基因相关miRNA的回收和鉴定的探针、试剂盒和方法
摘要：
本发明涉及了一种用于靶基因相关miRNA的回收和鉴定方法的探针，使用该探针的回收和鉴定方法以及试剂盒。具体涉及靶向p21基因mRNA的miRNA回收和鉴定的核酸探针，其具有SEQ ID NO:1的核苷酸序列。本发明提供了这种定点回收、鉴定miRNA的作用原理。本发明公开了这种新型回收、鉴定方法的用途和实施策略，特别是应用于细胞内特定基因RNA位点的miRNA的回收和鉴定。本发明证实这种定点回收技术可以特异性回收与p21基因mRNA相结合的miRNA，并证实这些miRNA可以调控p21基因的表达，该方法可以有效的回收、鉴定靶向特定基因mRNA的miRNA。
摘要（英）：

The invention relates to a method for recovering and identifying target gene-correlative miRNA, a kit and a method, concretely nucleic acid probe for recovering and identifying miRNA of target p21 gene mRNA, which has a nucleotide sequence of SEQ ID NO:1. The invention provides an action principle for recovering and indentifying the miRNA. The invention discloses application and an implementation strategy of the novel recovery and identification method, particularly recovery and identification of miRNA of specific gene RNA locus applied in a cell. The invention confirms that the locus recovery technology can specifically recover miRNA combined to mRNA of a p21 gene, and conforms that the miRNA can regulate and control p21 gene expression, and the method can effectively recovering and indentifying miRNA of the target specific gene mRNA.

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