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发明公开
CN103320530A 一种H1N1/H5N1型禽流感病毒检测试剂盒及其应用 失效 - 权利终止

一种H1N1/H5N1型禽流感病毒检测试剂盒及其应用
摘要：
本发明涉及生物检测技术领域，具体涉及一种利用双重荧光PCR法对禽流感病毒H1N1和H5N1在单管同时进行检测的引物、探针及试剂盒。本发明的H1N1/H5N1型禽流感病毒双重荧光PCR检测用引物及探针，其核苷酸序列如SEQIDNO：1-6所示；H1N1/H5N1型禽流感病毒双重PCR检测试剂盒，包括双重荧光PCR检测混合液，所述双重荧光PCR检测混合液中含有前述的引物和探针。利用本发明的试剂盒和引物探针能快速检测H1N1型禽流感病毒和H5N1型禽流感病毒，具有操作简便、灵敏度高、特异性好等优点；及时发现和确诊可疑病例，提高对该类疾病的监测水平。
摘要（英）：

The invention relates to the field of biological detection technology, and in particular relates to a primer, a probe and a kit for synchronously detecting avian influenza viruses H1N1 and H5N1 at a single tube. The nucleotide sequences of the primer and the probe for the double fluorescent PCR (polymerase chain reaction) detection of the H1N1/H5N1 type avian influenza virus are represented by SEQ ID NO: 1-6; the kit for the double fluorescent PCR detection of the H1N1/H5N1 type avian influenza virus comprises double fluorescent PCR detection mixed solution which contains the primer and the probe. The H1N1 type avian influenza virus and the H5N1 type avian influenza virus can be quickly detected by using the kit, the primer and the probe provided by the invention; the kit has the advantages of simple and convenient operation, high sensitivity, good specificity and the like; by adopting the kit, suspect cases can be found and be confirmed in time, the monitoring level to the disease is improved accordingly.

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