The invention relates to a frame synchronization method capable of resisting single-frequency interference. A sending end is used for adding frame synchronization sequence to a frame header of a physical layer frame, and a receiving end is used for setting a threshold value so as to judge whether a frame start position is detected. The method comprises the following steps: (1) passing a received signal through a low order normalized LMS (Least Mean Square) self-adapting filter; (2) subtracting the output of the low order normalized LMS self-adapting filter from the received signal, so as to obtain a corrected received signal; (3) performing mutual correlation computation of energy normalization to the corrected received signal and the local sequence, so as to obtain a correlation result of energy normalization; (4) comparing the obtained correlation result of energy normalization with the preset threshold value, so as to obtain the start position of the physical layer frame. The method can accurately estimate the start position of the physical layer frame in presence of great single-frequency interference. Therefore, the hardware resource cost is saved, and the system real-time performance is not affected.