The invention discloses a special partition board queen bee captivity device for bee overwintering, which can ensure that a queen bee cannot be starved and frozen to death in overwintering period. The device is formed by the steps as follows: a concave surface of a concave partition board provided with a frame, partition board lugs, queen bee partition holes, a queen bee inlet and outlet hole and queen bee inlet and outlet cover plate fixing holes is fixed into a concave surface of another concave partition board provided with a frame, partition board lugs, queen bee partition holes, a queen bee inlet and outlet hole and queen bee inlet and outlet cover plate fixing holes, and a queen bee inlet and outlet cover plate is covered and fixed by screws with 3 mm diameters. During bee overwintering period with unstable outside air temperatures and more than once cold wave or extreme low-temperature weather, the queen bee can always perform activities in the center of a bee cluster when the bee cluster is tightened on a honeycomb and move frequently, so that troubles brought by manual detection and movement of the queen bee captivity device are eliminated, the bee colony can live through the winter naturally and quietly, the service life of the bee is prolonged, the strength of the bee colony is maintained, the use is convenient and simple, and the service life is long.