The invention discloses a feed formula of taking cordyceps militaris culture medium residue as a material, and a preparation method thereof, and solves the problems that the traditional feed is high in cost, and the quality of eggs cannot be well improved. The feed formula disclosed by the invention is prepared from cordyceps militaris culture medium residue powder, corn flour, wheat bran, bean pulp, salt, fish meal, bone powder, vitamin mixture, microelements, methionine and premix compound. The invention also provides the preparation method of the Chicken feed formula. In the components of the feed formula, industrial waste, namely residual solid culture medium residue after cordyceps militaris sporocarp is picked up is utilized; the industrial waste is fully recycled, so that the production cost of the feed is greatly reduced.