The invention discloses a manufacturing method of a twisted optical fiber, which comprises the following steps of: 1) performing heating and first wiredrawing on an optical fiber perform to form a primary optical fiber of which the diameter is greater than that of the twisted optical fiber, wherein the optical fiber perform is in a non-rotation state in the first wiredrawing process; and 2) rotating the primary optical fiber, and performing heating and second wiredrawing on the primary optical fiber to form a twisted optical fiber; or keeping the primary optical fiber not rotating, performing heating and second wiredrawing on the primary optical fiber to form a secondary optical fiber, and rotating the secondary optical fiber to form a twisted optical fiber. The method disclosed by the invention greatly reduces the production difficulty, can guarantee the wiredrawing quality and the optical performance of the twisted optical fiber, and saves the cost.