利索-全球专利检索

  1. Login
  2. Sign Up
Invention Publication
CN103361436A 耐除草剂转基因棉花MON1445的转化体特异性引物、探针及在实时荧光PCR检测中的应用 无效 - 驳回

耐除草剂转基因棉花MON1445的转化体特异性引物、探针及在实时荧光PCR检测中的应用
Abstract:
本发明涉及耐除草剂转基因棉花MON1445的转化体特异性引物、探针及在实时荧光PCR检测中的应用，引物与探针序列如SEQ ID No.1，SEQ ID No.2，SEQ ID No.3所示，提取待检测样品基因组DNA加入引物扩增可以进行SYBR Green I实时荧光PCR检测，提取待检测样品基因组DNA，加入引物与探针组合扩增进行Taqman探针实时荧光PCR检测。本发明设计的引物与探针序列应用于PCR检测灵敏、准确、简单、可靠。
Abstract(English):

The invention relates to transformant specific primers and probes of herbicide-tolerant transgenic cotton MON1445, and an application in real-time fluorescence PCR detection. The sequences of the primers and the probes are shown in SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3, the genome DNA of a to-be detected sample is extracted and added with primers for amplification then for SYBR Green I real-time fluorescence PCR detection; and the genome DNA of the to-be detected sample is extracted and added with combination of the primers and the probes for amplification then for Taqman probe real-time fluorescence PCR detection. The designed primer and probe sequences of the invention are applied to PCR detection, and the detection is sensitive, accurate, simple and realiable.

Patent Agency Ranking
0/0