The invention discloses an auto-control high pressure crude oil sampling device. The auto-control high pressure crude oil sampling device comprises an electric actuator, an automatic controller, a weight sensor, a sampling shell, a gland, a sampling port, a sealing plug and a transmission rod. The auto-control high pressure crude oil sampling device is characterized in that the sampling shell is cylindrical, the center of the bottom of the sampling shell is provided with an oil inlet hole, the inner circumference of the sampling shell is provided with a female thread, and the lower part of the middle circumference of the sampling shell is provided with the sampling port; the female thread is provided with the sealing plug which is in thread fit, the top of the female thread is provided with the gland which is in flange connection or screwed connection, the upper part of the gland is provided with a pressing cap which is in threaded connection, and a pressure pad and a sealing packing are arranged between the gland and the pressing cap; the center of the sealing plug is fixedly provided with the cylindrical transmission rod, and the transmission rod passes through the central holes of the gland, the pressure pad and the pressing cap so as to be connected with the electric actuator; the lower part of the sampling port is provided with the weight sensor, and a control circuit of the electric actuator and an execution circuit of the weight sensor are connected with a control circuit of the automatic controller.