The invention provides an experimental ability quantitative evaluation method based on an experiment resource tree. The method comprises the following steps of traversing experiment resources; selecting an experiment process; building an experiment resource tree; and determining a quantitative evaluation value. According to the experimental ability quantitative evaluation method based on the experiment resource tree, various resources used in the experiment process are combined in a form of a tree and are subjected to weighing calculation, so that the strength of the experiment ability is judged, and the quantitative evaluation problems of comparison between laboratories and resource distribution are solved.