The invention discloses a unidirectional cross-security subzone transformer substation comprehensive measurement and control platform. The platform comprises a security zone 3 and a security zone 1; and the security zone 3 is connected with the security zone 1 through a data forward isolation device. The invention discloses a unidirectional cross-security subzone transformer substation comprehensive measurement and control method. By the method, the intelligent monitoring of substation operation equipment is realized by transmitting data from the security zone 1 to the security zone 3 through the data forward isolation device. The invention provides a unified monitoring platform on the basis of a data unidirectional transmission isolation technology, a substation application protocol matching and analyzing technology, a real-time data response technology, a substation operation equipment intelligent remote control technology and a substation operation equipment intelligent monitoring technology for solving the problems of external network control vulnerability which damages substation equipment non-permissively and intentionally, the closure of substation equipment monitoring data, the staleness of detection data, the oneness of intelligent remote control and the like.