The invention belongs to an optimization method of an efficient exogenous-gene transformation vector, and the optimization method belongs to the field of plant molecular biology and plant gene engineering. The method comprises: building one segment of a nuclear matrix attachment region (MAR) sequence separated from tobacco into two ends of a GUS reporter-gene expression cassette in the plant expression vector, guiding into paddy rice and tobacco via an agrobacterium mediated method. According to detections, compared with the paddy rice and the tobacco without MAR introduction, the paddy rice and the tobacco each introduced with MAR are respectively improved by 9 times in GUS protein expression level and correspondingly improved by 40% in transformation efficiency, and also the plants introduced with MAR is improved by 30% in hereditary stability of exogenous gene among progeny individuals. The optimization method is capable of effectively improving transformation efficiency of exogenous gene in the plants and hereditary stability in transgenic progeny, so that efficient and stable transformation is realized.